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EIF2A Antibody / Eukaryotic translation initiation factor 2A

CAT: 0800-FY13068Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-FY13068Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
Q9BY44
Host
Rabbit
Reactivity
Human, Rat
Immunogen
E.coli-derived human EIF2A recombinant protein (Position: H24-I585) was used as the immunogen for the EIF2A antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This EIF2A antibody is available for research use only.
Storage Conditions
After reconstitution, the EIF2A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the EIF2A antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Immunohistochemical staining of EIF2A using anti-EIF2A antibody. EIF2A was detected in a paraffin-embedded section of human pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-EIF2A antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

UniProtKB · Q9BY44

Eukaryotic translation initiation factor 2A

EIF2A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BY44
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EIF2A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
eIF-2A
EC number
—
Processing
—
Secondary accessions
A8MPS6, B4DF96, B4DQ14, D3DNI9, Q5QTR2, Q7Z4E9, Q8NFM1, Q96EW9, Q96K81
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Domain

Coiled coilRepeatWD repeat

Molecular function

Initiation factor

Biological process

Protein biosynthesisTranslation regulation

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