CD20 Antibody

• Description :

  This antibody recognizes a protein of 33-37kDa, identified as CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

• Specifications :

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt :

  P11836

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Human tonsil B cells were used as the immunogen for this CD20 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  SPM494

• Applications :

  IHC-P

• Format :

  Bioreactor concentrate

• Buffer :

  1X PBS, pH 7.4

• Limitations :

  This CD20 antibody is available for research use only.

• Storage Conditions :

  CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation :

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• Location :

  Predominantly cell surface with some cytoplasmic

• Image Legend :

  IHC testing of CD20 antibody and FFPE human lymphoma tissue (clone SPM494) .