Cleaved-Notch1 Rabbit Polyclonal Antibody

• Background:

  Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.

• Product Name Alternative:

  NOTCH1; TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1

• Gene Name:

  NOTCH1

• Gene ID:

  4851

• Swiss Prot:

  P46531

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, ICC/IF, ELISA

• Field of Research:

  Neuroscience

• Purification:

  Affinity Purification

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100, ICC/IF 1:50-1:200, ELISA 1:5000-1:20000

• Form:

  Liquid

• Buffer:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Modification:

  Cleaved

• Molecular Weight:

  Calculated MW: 273 kDa; Observed MW: 110 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

• Isotype:

  IgG