Integrin alpha 4 Recombinant Antibody [Natalizumab] (OAAV00403)

• Gene Name:

  CD40 molecule

• Gene Aliases:

  B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDW40; p50; TNFRSF5; tumor necrosis factor receptor superfamily member 5.

• Gene ID:

  958

• Swiss Prot:

  P25445

• Accession Number:

  NP_001241.1

• Reactivity:

  Human

• Immunogen:

  A54 human anti-CD40 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-FcGamma fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.

• Target:

  This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

• Clonality:

  Recombinant Monoclonal

• Clone:

  A54 scFv

• Type:

  Recombinant Monoclonal Antibody

• Purification:

  Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution:

  Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

• Format:

  PBS with 0.02% Proclin 300.

• Reconstitution:

  Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

• Fragment:

  IgG, lambda

• Specificity:

  A54 binds specifically to the D1 domain of CD40 (and can only bind to the intact i.e. non-truncated version of CD40 and when an AD2 peptide tag has not been added) and can recognise CD40 on a B cell line (BJAB) . This scFv binds to CD40 (immobilized by amine coupling) with a KD within the range of 40nM. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40 binds to the membrane protein CD154 of the TNF superfamily and this interaction is required for the seconfary immune responses and germinal centre formation.

• NCBI Gene Symbol:

  CD40

• Host or Source:

  Rabbit

• Protein Name:

  Tumor necrosis factor receptor superfamily member 5

• Gene Name URL:

  CD40

• Nucleotide Accession Number:

  NM_001250.5