Products for Research Use Only

Lymphoprep

CAT: 877-1858-01Size: 500 mLDry Ice: NoHazardous: No
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CAT#:877-1858-01Size:500 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Lymphoprep™ is a high-quality density gradient separation medium developed for the isolation and purification of human mononuclear cells from whole blood samples. The optimized formulation enables the separation of lymphocytes and monocytes from other blood components, including erythrocytes and granulocytes, through centrifugation based on differences in cellular density. Lymphoprep™ provides researchers with a convenient ready-to-use solution for preparing purified mononuclear cell fractions while maintaining cell integrity for downstream applications.
Specifications
Lymphoprep™ Density Gradient Medium is supplied as a ready-to-use sterile solution designed for the isolation of human peripheral blood mononuclear cells (PBMCs). This product is provided in a 500 mL format and has a density of 1.077 ± 0.001 g/mL, allowing efficient separation of lymphocytes and monocytes from whole blood samples during density gradient centrifugation. The formulation is tested for quality and suitability in research workflows requiring reliable preparation of mononuclear cell populations. Lymphoprep™ is intended for research use only (RUO) and is suitable for applications in immunology, cell biology, molecular biology and cellular analysis.
Applications
Peripheral Blood Mononuclear Cell (PBMC) Isolation

Lymphoprep™ is commonly used for the preparation of purified mononuclear cell fractions from human blood samples.

Applications include:
  • PBMC isolation
  • Lymphocyte preparation
  • Monocyte enrichment
  • Immune cell recovery
  • Immunology Research

Lymphoprep™ supports research applications including:


  • Immune cell profiling
  • T-cell studies
  • Flow cytometry analysis
  • Functional immune assays
  • Cell Biology Research

Used for preparation of cellular populations for:
  • Cell characterization
  • Cellular phenotyping
  • Cell-based assays
  • Primary cell research
  • Molecular Biology Applications

Purified mononuclear cells obtained using Lymphoprep™ can be used for:

  • DNA extraction
  • RNA isolation
  • Gene expression studies
  • Single-cell analysis workflows
  • Separation Principle
  • How Lymphoprep™ Works

Lymphoprep™ separates blood components according to their buoyant density during centrifugation.

After centrifugation:

  • Plasma remains at the upper layer
  • Mononuclear cells accumulate at the plasma–medium interface
  • Granulocytes and erythrocytes migrate through the density barrier

This enables efficient recovery of enriched PBMC populations for further laboratory analysis.
Field of Research
Lymphoprep™ is widely used in:

  • Immunology
  • Cell biology
  • Cancer research
  • Vaccine research
  • Infectious disease research
  • Cellular therapy research
  • Single-cell research
Sample Type
Lymphoprep™ can be used with:
  • Human whole blood
  • Peripheral blood samples
  • Buffy coat preparations
  • Mononuclear cell samples
Features & Benefits
✓ Ready-to-use density gradient medium ✓ Optimized for PBMC isolation ✓ Efficient lymphocyte and monocyte separation ✓ Sterile formulation ✓ Consistent density performance ✓ Supports high-quality cell recovery ✓ Compatible with downstream cellular analysis ✓ Suitable for immunology and biomedical research workflows

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