Lymphoprep

CAT: 877-1856-02Size: 4x 250 mLDry Ice: NoHazardous: No

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CAT#:877-1856-02Size:4x 250 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Lymphoprep™ is a ready-to-use, sterile density gradient medium developed for the efficient isolation of human peripheral blood mononuclear cells (PBMCs) including lymphocytes and monocytes. Based on a polysaccharide and sodium diatrizoate formulation, Lymphoprep™ provides a defined density gradient for the separation of mononuclear cells from whole blood samples using centrifugation. Its optimized formulation enables rapid and reliable cell recovery while maintaining cell integrity for downstream research applications. Lymphoprep™ is widely used in immunology, cell biology, flow cytometry, cell therapy research and molecular biology workflows requiring purified mononuclear cell populations.

Specifications

Lymphoprep™ is supplied as a ready-to-use sterile density gradient solution designed for the isolation of human mononuclear cells from peripheral blood samples. The medium has a density of 1.077 ± 0.001 g/mL and is formulated to allow efficient separation of lymphocytes and monocytes from erythrocytes and granulocytes during density gradient centrifugation. Lymphoprep™ is endotoxin-tested and intended for research applications requiring reliable preparation of purified mononuclear cell fractions.

Applications

Peripheral Blood Mononuclear Cell (PBMC) Isolation

Lymphoprep™ is commonly used for the preparation of purified mononuclear cell fractions from human blood samples.

Applications include:

PBMC isolation
Lymphocyte preparation
Monocyte enrichment
Immune cell recovery

Immunology Research

Lymphoprep™ supports research applications including:

Immune cell profiling
T-cell studies
Flow cytometry analysis
Functional immune assays

Cell Biology Research

Used for preparation of cellular populations for:

Cell characterization
Cellular phenotyping
Cell-based assays
Primary cell research

Molecular Biology Applications

Purified mononuclear cells obtained using Lymphoprep™ can be used for:

DNA extraction
RNA isolation
Gene expression studies
Single-cell analysis workflow

Field of Research

Lymphoprep™ is widely used in:

Immunology
Cell biology
Cancer research
Vaccine research
Infectious disease research
Cellular therapy research
Single-cell research

Assay Principle

Lymphoprep™ separates blood components according to their buoyant density during centrifugation.

After centrifugation:

Plasma remains at the upper layer
Mononuclear cells accumulate at the plasma–medium interface
Granulocytes and erythrocytes migrate through the density barrier

This enables efficient recovery of enriched PBMC populations for further laboratory analysis.

Sample Type

Lymphoprep™ can be used with:

Human whole blood
Buffy coat samples
Peripheral blood samples
Mononuclear cell preparations

Features & Benefits

✓ Ready-to-use density gradient medium ✓ Designed for PBMC isolation ✓ Suitable for lymphocyte and monocyte separation ✓ Sterile formulation ✓ Endotoxin tested ✓ Provides high-quality mononuclear cell recovery ✓ Compatible with immunology and cell biology workflows ✓ Suitable for downstream applications including flow cytometry and molecular analysis

Alternative Products

Catalog	Name
M1274-250	Lymphoprep™
AXS-1114544	Lymphoprep
1858-1	LymphoPrep™ 1 x 500 mL
1856-1	LymphoPrep™ 1 x 250 mL
1858-6	LymphoPrep™ 6 x 500 mL
1856-4	LymphoPrep™ 4 x 250 mL
18001	LymphoPrep™ Tubes 18 x 10 mL