Dolphin CXCL10 (IP-10) ELISpot

• Background:

  CXCL10, also known as Interferon gamma-induced protein 10 or small-inducible cytokine B10, is a member of the C-X-C chemokine family. CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. There have been 17 different C-X-C chemokines described in mammals, that are subdivided into two categories, those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the C-X-C motif (ELR-positive), and those without an ELR motif (ELR-negative) . ELR-positive C-X-C chemokines specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. C-X-C chemokines that lack the ELR motif are chemoattractant for lymphocytes. CXCL10 elicits its effects by binding to the cell surface chemokine receptor CXCR3.

• Description:

  Dolphin CXCL10 ELISpot contains Capture Antibody, Standard Control, and Detection Antibody for development of up to two (2) ELISpot assays. Other required materials and reagents are not supplied. The antibodies have been determined to function in an ELISpot with the Standard Control provided. Components may also be purchased separately. For research use only.

• Applications:

  ELISpot

• Homology:

  Delphinapterus leucas (beluga whale), Globicephala melas (long-finned pilot whale), Lagenorhynchus obliquidens (Pacific white-sided dolphin), Neophocaena asiaeorientalis asiaeorientalis (Yangtze finless porpoise), Orcinus orca (killer whale), Phocoena sinus (vaquita), Tursiops truncatus (common bottlenose dolphin)

• Storage Temperature:

  2°C to 8°C