Mouse IFN lambda 3 (IL-28B) ELISpot

• Background:

  IL-28B, IL-28A, and IL-29 are three closely related IFN-lambda cytokines that can be induced by viral infection that induce signaling through the Jak/STAT pathway. IL-28B, and other IFN-lambda cytokines, are well established as having several antiviral and anti-cancer activities. IFN-lambda, restricts virus from spreading into the brain and plays a major role in chronic viral infections found in the gastrointestinal tract. IL-28B, like other IFN-lambda cytokines, modulates innate and adaptive immunity, autoimmunity, and tumor progression. Unlike type I IFNs, which are able to stimulate most cell types, IL-28B, along with the other IFN-lambda cytokines, stimulation appears to be limited to dendritic cells and some tumor cell lines. Due to the affect IL-28B has on the adaptive immune system, it has gained much interest as an immunotherapeutic agent for treatment of allergic asthma. The IL-28 cytokines control T cell responses in vivo through the modulation of lung CD11c (+) DC function in experimental allergic asthma animal models. IFN-lambda cytokines, including IL-28B, have been shown to interact with a heterodimeric, class II cytokine receptor that consists of the IL-10 receptor, beta (IL-10RB) and the IL-28 receptor, alpha (IL-28RA) subunits. The promiscuous IL-10RB subunit is also common to IFN-alpha/beta family members, but the IL-28RA subunit is specific for the IFN-lambda cytokines, IL-28B, IL-28A, and IL-29.

• Description:

  Mouse IL-28B ELISpot contains Capture Antibody, Standard Control, and Detection Antibody for development of up to two (2) ELISpot assays. Other required materials and reagents are not supplied. The antibodies have been determined to function in an ELISpot with the Standard Control provided. Components may also be purchased separately. For research use only.

• Applications:

  ELISpot

• Homology:

  Mus musculus (house mouse)

• Storage Temperature:

  2°C to 8°C