4E-BP1 (Phospho-Thr70) Antibody

• Background:

  4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

• Description:

  This is a 4E-BP1 (Phospho-Thr70) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  4EBP1; P/OKCL.6; P/OKCL.6; PHAS-I

• Gene ID:

  1978

• Swiss Prot:

  Q13541

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of threonine70 (T-K-T (p) -P-P) derived from Human 4E-BP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  IHC

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  IHC 1:50-1:100, WB 1:500-1:1000

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Observed Molecular Weight:

  12kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG