MARCKS (Phospho-Ser158) Antibody

• Background:

  MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

• Description:

  This is a MARCKS (Phospho-Ser158) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  MACS; MARCS; PKCSL; PRKCSL; Protein kinase C substrate

• Gene ID:

  4082

• Swiss Prot:

  P29966

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Peptide sequence around phosphorylation site of serine 158 (R-F-S (p) -F-K) derived from Human MARCKS.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IF

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IF 1:100-1:200

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG