4E-BP1 (Phospho-Thr37) Antibody

• Background:

  Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

• Description:

  This is a 4E-BP1 (Phospho-Thr37) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  BP-1;4EBP1;4E-BP1; PHAS-I

• Gene ID:

  1978

• Swiss Prot:

  Q13541

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Peptide sequence around phosphorylation site of threonine 37 (S-T-T (p) -P-G) derived from Human 4E-BP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Observed Molecular Weight:

  18kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG