P27Kip1 (Phospho-Thr187) Antibody

• Background:

  Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

• Description:

  This is a p27Kip1 (Phospho-Thr187) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; KIP1

• Gene ID:

  1027

• Swiss Prot:

  P46527

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of threonine 187 (E-Q-T (p) -P-K) derived from Human p27Kip1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG