TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag)

• Description:

  Tumor necrosis factor receptor superfamily, member 17 (TNFRSF17), also known as B cell maturation antigen (BCMA) or CD269 antigen, is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13BBAFF), and to lead to NF-kappaB and MAPK8/JNK activation. TNFRSF17/BCMA/CD269 also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. TNFRSF17/BCMA/CD269 is a receptor for TALL-1 and BCMA activates NF-kappaB through a TRAF5-, TRAF6-, NIK-, and IKK-dependent pathway. The identification of TNFRSF17 as a NF-kappaB-activating receptor for TALL-1 suggests molecular targets for drug development against certain immunodeficient or autoimmune diseases. TNFRSF17/BCMA is a target of donor B-cell immunity in patients with myeloma who respond to DLI. Antibody responses to cell-surface BCMA may contribute directly to tumor rejection in vivo.

• Synonyms:

  BCM Protein; BCMA Protein; CD269 Protein; TNFRSF13A Protein

• Accession Number:

  NP_001183.2

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  His-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

• Bioactivity:

  1. Immobilized Human BAFF at 2μg/mL (100 μL/well) can bind Human BCMA. The EC50 of Human BCMA is 30-80 ng/mL. 2. Immobilized Mouse TNFSF13 at 2μg/mL (100 μL/well) can bind Human BCMA. The EC50 of Human BCMA is 0.6-2.1 ng/mL. 3. 293 cells were transduced with anti-BCMA-scFv and subjected to flow cytometric analysis using recombinant human BCMA and a negative control protein. The cells were then stained with a PE-conjugated anti-His antibody. Non-transduced 293 cells were used as a control. 4. Loaded Recombinant Human BAFF / BLyS / TNFSF13B Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human BCMA Protein, His Tag with an affinity constant of 0.267 μM as determined in BLI assay (Sartorius Octet RED384) . 5. Immobilized Anti-BCMA Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, His Tag) . The EC50 is 3-10 ng/mL. 6. Anti-BCMA Antibody, Human IgG1 captured on Protein A chip can bind Recombinant Human BCMA Protein, His Tag with an affinity constant of 22.3 nM as determined in an SPR assay (Biacore 8K) .

• Length:

  65

• Form:

  Lyophilized

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  17 kDa (glycosylated)

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells