R-Spondin-1

• Description:

  R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in the adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Recombinant Murine R-Spondin-1 is a 27.1 kDa protein consisting of 245 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions.

• Synonyms:

  Roof plate-specific Spondin-1, RSPO1

• NCBI Gene ID:

  192199

• UniProt:

  Q9Z132

• Accession Number:

  NP_619624.2

• Accession Number mRNA:

  NM_138683.2

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCQEGLYL HKGRCYPACP EGSTAANSTM ECGSPAQCEM SEWSPWGPCS KKRKLCGFRK GSEERTRRVL HAPGGDHTTC SDTKETRKCT VRRTPCPEGQ KRRKGGQGRR ENANRHPARK NSKEPGSNSR RHKGQQQPQP GTTGPLTSVG PTWAQ

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Bioactivity:

  R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells.

• Length:

  245

• Form:

  Lyophilized

• Buffer:

  10mM Sodium Phosphate, pH 7.5 and 150mM NaCl

• Reconstitution:

  Water

• Molecular Weight:

  40.0 kDa

• Host or Source:

  CHO cells