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Resistin antagonist

CAT: 0209-500-093-L100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0209-500-093-L100Size:100 µg
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Description
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins (monomeric peptide contains 11 cysteine residues) referred to as the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) or FIZZ3 (Found in Inflammatory Zone 3) . Human resistin antagonist is a monomeric C7A mutant that does not form covalent dimers. It is expressed and purified by chromatographic techniques.
Synonyms
Adipose tissue-specific secretory factor (ADSF), C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3
NCBI Gene ID
56729
UniProt
Q9HD89
Accession Number
NP_065148
Accession Number mRNA
NM_020415
Reactivity
Human
Cross Reactivity
Mouse, Human
Sequence
ASSKTLCSMEE AINERIQEVA GSLIFRAISS IGLECQSVTS RGDLATCPRG FAVTGCTCGS ACGSWDVRAE TTCHCQCAGM DWTGARCCRV QP
Assay Protocol
It is recommended to reconstitute the lyophilized resistin in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other buffered aqueous solutions containing carrier proteins.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
>98.0% as determined by RP-HPLC, SDS-PAGE Silver Stained and Gel filtration.
Bioactivity
The biological activity was evidenced by resistin antagonist activity to inhibit resistin-induced Akt phosphorylation in two cell lines. It also reduced the weight (mainly the visceral fat) and normalized GTT and ITT inHFD-fed mice.
Length
93
Form
Lyophilized
Reconstitution
Water
Molecular Weight
9.7 kDa
Host or Source
E. coli
N Terminal Sequence
ASSKTLA

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