Leptin (animal-free)

• Description:

  Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant Rat Leptin is a 16.2 kDa protein containing 147 amino acid residues.

• Synonyms:

  Lep; ob; obese

• NCBI Gene ID:

  16846

• UniProt:

  P41160

• Accession Number:

  NP_032519.1

• Accession Number mRNA:

  NM_008493.3

• Chromosomal Location:

  6 A3.3; 6 10.5 cM

• Reactivity:

  Mouse

• Cross Reactivity:

  Hamster, Human, Pig, Rat, Mouse

• Sequence:

  MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Murine Leptin has been shown to be biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight for 14 days. Significant effects on body weight , food consumption, and plasma glucose levels were observed to saline-treated controls.

• Length:

  147

• Form:

  Lyophilized

• Molecular Weight:

  16.2 kDa

• Host or Source:

  E. coli