Leptin (animal-free)

• Description:

  Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant human Leptin is a 16.0 kDa protein containing 147 amino acid residues.

• Synonyms:

  LEP; OB; OBS

• NCBI Gene ID:

  3952

• UniProt:

  P41159

• Accession Number:

  NP_000221.1

• Accession Number mRNA:

  NM_000230

• Chromosomal Location:

  7q31.1

• Reactivity:

  Human

• Cross Reactivity:

  Chicken, Monkey, Mouse, Rat, Human

• Sequence:

  MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Human Leptin is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.

• Length:

  147

• Form:

  Lyophilized

• Molecular Weight:

  16 kDa

• Host or Source:

  E. coli