IL-6

• Description:

  IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R α and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant murine IL-6 is a 21.7 kDa protein containing 187 amino acid residues.

• Synonyms:

  Il6; Il-6

• NCBI Gene ID:

  16193

• UniProt:

  P08505

• Accession Number:

  NP_112445.1

• Accession Number mRNA:

  NM_031168

• Chromosomal Location:

  5 B1; 5 17.0 cM

• Reactivity:

  Mouse

• Cross Reactivity:

  Monkey, Mouse, Rat, Trout

• Sequence:

  MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & Coomassie stain

• Bioactivity:

  The ED50 as determined by the dose-dependent stimulation of murine hybridoma B9 cells is in the range of 5-20 pg/ml.

• Length:

  188

• Form:

  Lyophilized

• Buffer:

  50 mM Acetic Acid

• Reconstitution:

  50 mM Acetic Acid

• Molecular Weight:

  21.7 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  MFPTS