G-CSF

• Description:

  G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and mouse G-CSF are cross-species reactive. Recombinant murine G-CSF is a 19.0 kDa protein consisting of 179 amino acid residues.

• Synonyms:

  Csf3; Csfg; G-CSF; MGI-IG

• NCBI Gene ID:

  12985

• UniProt:

  P09920

• Accession Number:

  NP_034101.1

• Accession Number mRNA:

  NM_009971

• Chromosomal Location:

  11 D; 11 57.0 cM

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse, Rat

• Sequence:

  MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

• Length:

  179

• Form:

  Lyophilized

• Molecular Weight:

  19.0 kDa

• Host or Source:

  E. coli