FGF8a / FGF-8a Protein

• Description:

  In mammalian embryos, transient Fgf8 expression defines the developing isthmic region, lying between the midbrain and the first rhombomere, but there has been uncertainty about the existence of a distinct isthmic segment in postnatal mammals. Retinoic acid (RA) directly represses Fgf8 through a RARE-mediated mechanism that promotes repressive chromatin, thus providing valuable insight into the mechanism of RA-FGF antagonism during progenitor cell differentiation. Fgf8 encodes a key signaling factor, and its precise regulation is essential for embryo patterning.

• Synonyms:

  AIGF Protein; FGF-8 Protein; FGF8 Protein; HBGF-8 Protein; HH6 Protein; KAL6 Protein

• Accession Number:

  NP_149355.1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Bioactivity:

  Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.8-3.3 μg/mL.

• Length:

  183

• Form:

  Lyophilized

• Buffer:

  20 mM Tris, 500 mM NaCl, pH 8.5.

• Reconstitution:

  See Datasheet

• Molecular Weight:

  21.3 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  E.coli