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FGF-7/KGF

CAT: 0209-100-163-L50Size: 50 µgDry Ice: NoHazardous: No
CAT#:0209-100-163-L50Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Keratinocyte Growth Factor (KGF/FGF-7) is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of variety of tissues, by promoting cellular proliferation and differentiation. KGF/FG-7 is a mitogen factor specific for epithelial cells and keratinocytes and signals through FGFR 2b. KGF/FGF-7 plays a role in kidney and lung development, angiogenesis, and wound healing. Recombinant human KGF/FGF-7 is an 18.9 kDa protein consisting of 163 amino acid residues.
NCBI Gene ID
2252
UniProt
P21781
Accession Number
NP_002000
Accession Number mRNA
NM_002009
Chromosomal Location
15q21.2
Reactivity
Human
Cross Reactivity
Mouse, Rat, Human, Leech
Sequence
CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT
Assay Protocol
Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 95% by SDS-PAGE & HPLC analyses
Bioactivity
The expected ED50 as determined by a cell proliferation assay using 4MBr-5 cells is 0.1-15 ng/ml.
Length
163
Form
Lyophilized
Buffer
15mM Sodium Phosphate, pH 7.0 + 25mM NaCl
Reconstitution
PBS
Molecular Weight
18.9 kDa
Storage Conditions
The lyophilized protein is stable at room temperature for 3 weeks should be stored desiccated below -18°C. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
Host or Source
E. coli

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