FGF4 Protein

• Description:

  FGF-4 (fibroblast growth factor 4), also known as K-FGF (Kaposi’s sarcoma associated FGF), is a 25 kDa secreted, heparin binding member of the FGF family. The human FGF-4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C terminus. Mature human FGF-4 shares a high aa identity with mouse, rat, canine and bovine FGF-4, respectively. The expression of FGF-4 and its receptors, FGF-R1c, -R2c, -R3c and R4, is spatially and temporally regulated during embryonic development. Its expression in the mouse trophoblast inner cell mass promotes expression of FGF-R2, and is required for maintenance of the trophectoderm and primitive endoderm. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in-vitro. FGF-4 is mitogenic for fibroblasts and endothelial cells in-vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in-vivo and has been investigated as therapy for coronary artery disease. Recombinant human FGF-4 has a molecular weight of 16.92 kDa and consists of 153 amino acid residues (Ser54-Leu206) .

• Synonyms:

  FGF4, Heparin secretory-transforming protein 1, HST, HST-1, HSTF-1, Heparin-binding growth factor 4, HBGF-4, Transforming protein KS3, KFGF

• Accession Number:

  NP_001998.1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Assay Protocol:

  Centrifuge the vial prior to opening. Do not vortex. The lyophilized FGF-4 should be reconstituted in sterile water to a concentration of 0.25 mg/mL. Store at 2 °C to 8 °C for up to 1 week or prepare for extended storage.

• Endotoxin:

  < 0.1 EU per μg of the protein

• Purity:

  ≥ 95 % as determined by SDS-PAGE and SEC-HPLC

• Bioactivity:

  Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.04-0.2

• Length:

  153

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, pH 7.4

• Additives:

  5 % trehalose + 5 % mannose + 0.01 % tween-80

• Reconstitution:

  Centrifuge the vial prior to opening. Do not vortex. The lyophilized FGF-4 should be reconstituted in sterile water to a concentration of 0.25 mg/mL. Store at 2 °C to 8 °C for up to 1 week or prepare for extended storage.

• Molecular Weight:

  16.92 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  E.coli

• N Terminal Sequence:

  Met