FGF-20

• Description:

  FGF-20 is secreted heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-20 signals through the FGFR 2c and 3c and is expressed during limb and brain development. Recombinant human FGF-20 is a 23.2 kDa protein containing 209 amino acid residues.

• Synonyms:

  FGF20; FGF-20

• NCBI Gene ID:

  26281

• UniProt:

  Q9NP95

• Accession Number:

  NP_062825.1

• Accession Number mRNA:

  NM_019851

• Chromosomal Location:

  8p22

• Reactivity:

  Human

• Cross Reactivity:

  Monkey, Mouse

• Sequence:

  PLAEVGGFLG GLEGLGQQVG SHFLLPPAGE RPPLLGERRS AAERSARGGP GAAQLAHLHG ILRRRQLYCR TGFHLQILPD GSVQGTRQDH SLFGILEFIS VAVGLVSIRG VDSGLYLGMN DKGELYGSEK LTSECIFREQ FEENWYNTYS SNIYKHGDTG RRYFVALNKD GTPRDGARSK RHQKFTHFLP RPVDPERVPE LYKDLLMYT

• Assay Protocol:

  Reconstitute in 10mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 90% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.

• Length:

  209

• Form:

  Lyophilized

• Buffer:

  25mM Tris, pH 7.6 + 1.5M NaCl

• Reconstitution:

  25mM Tris, pH 7.6

• Molecular Weight:

  23.2 kDa

• Host or Source:

  E. coli