FGF acidic

• Description:

  FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGF-acidic is a 15.8 kDa protein consisting of 140 amino acid residues.

• Synonyms:

  FGF2; BFGF; FGFB; HBGF-2

• NCBI Gene ID:

  2246

• UniProt:

  P05230

• Accession Number:

  NP_000791.1

• Accession Number mRNA:

  NM_000800.4

• Chromosomal Location:

  5q31

• Reactivity:

  Human

• Cross Reactivity:

  Cow, Mouse, PIG, Rat, Human, Hamster, Leech

• Sequence:

  MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/ml. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.5 ng/ml in the presence of 10 μg/ml heparin.

• Length:

  141

• Form:

  Lyophilized

• Molecular Weight:

  15.8 kDa

• Host or Source:

  E. coli