CD137 / 4-1BB Protein (ECD, hFc Tag)

• Description:

  CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials. Soluble forms of CD137 (sCD137) are generated by differential splicing. sCD137 can bind to CD137 ligand to antagonize the costimulatory activities of the membrane-bound CD137 and reduce T cell proliferation and IL-2 secretion.

• Synonyms:

  4-1BB Protein; CD137 Protein; CDw137 Protein; ILA Protein; IMD109 Protein

• Accession Number:

  NP_001552.2

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  HFc-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

• Bioactivity:

  1.Measured by its binding ability in a functional ELISA. Immobilized TNFRSF9-Fc at 10 μg/mL (100 μL/well) can bind TNFSF9/Biotin, The EC50 of TNFSF9/Biotin is 20-60 ng/mL. 2.Loaded Biotinylated Recombinant Human 4-1BB Ligand/TNFSF9 Protein, Fc Tag on SA Biosensor, can bind Recombinant Human 4-1BB/CD137 Protein, hFc Tag with an affinity constant of 4.29 nM as determined in BLI assay (Sartorius Octet RED384) .

• Length:

  401

• Form:

  Lyophilized

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  58.9 kDa (glycosylated)

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells