Recombinant Phospho-Vimentin (Ser39) Monoclonal Antibody

• Background:

  Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA) and inhibited by the antiinflammatory cytokine IL10, suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

• Abbreviation:

  Phospho-Vimentin (Ser39)

• UniProt:

  P08670

• Host:

  Rabbit

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  Phosphorylated human Vimentin (Ser39) peptide

• Target:

  HEL; Epididymis luminal protein; CTRCT; CTRCT30; HEL113; Vimentin; VIM; Epididymis luminal protein 113; VIME; FLJ36605; epididymis secretory sperm binding protein; CTRCT30; HEL113; vimentin; CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME; Vimentin

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A820

• Conjugation:

  Unconjugated

• Applications:

  WB

• Field of Research:

  Neuroscience; Signal Transduction; Stem Cells; Cancer; Developmental Biology

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:1000

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  54 kDa

• Observed Molecular Weight:

  56 kDa

• Isotype:

  IgG, κ