Recombinant Non-Phospho (Active) β-Catenin (Ser33, Ser37, Thr41) Monoclonal Antibody

• Background:

  Β-catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.Non-phospho (Active) β-Catenin (Ser33/37/Thr41) Rabbit mAb specifically recognizes β-catenin that is non-phosphorylated at Ser33, Ser33 or Thr41. Non-phospho β-Catenin (Ser33, Ser33 or Thr41) protein is useful as a readout of stabilized β-catenin protein, and therefore functionally active in cell-cell adhesion and mediating transcriptional activity via the canonical Wnt signaling pathway.

• Abbreviation:

  Non-Phospho (Active) β-Catenin (Ser33, Ser37, Thr41)

• UniProt:

  P35222

• Host:

  Rabbit

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  Recombinant human Non-phospho (Active) β-Catenin (Ser33/37/Thr41) fragment

• Target:

  MRD; PRO; Catenin beta; SW-cl; OK/SW-cl; CTNNB; MRD19; armadillo; beta Catenin; CTNNB1; Beta-catenin; Catenin beta-1; OK; SW-cl.35; OK/SW-cl.35; PRO2286;88kDa; beta 1; Cadherin associated protein; Catenin (cadherin associated protein) ; Catenin beta 1; CATNB; CHBCAT; CTNB1; DKFZp686D02253; FLJ25606; FLJ37923; OTTHUMP00000162082; OTTHUMP00000165222; OTTHUMP00000165223; OTTHUMP00000209288; OTTHUMP00000209289

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A593

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC; IF

• Field of Research:

  Signal Transduction; Neuroscience; Stem Cells; Cancer; Cardiovascular

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:1000; IHC 1:100-1:500; IF 1:50

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  85 kDa

• Observed Molecular Weight:

  100 kDa

• Isotype:

  IgG, κ