Recombinant PRMT5 Monoclonal Antibody

• Background:

  Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3) ; such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H and may regulate its transcriptional elongation properties. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

• Abbreviation:

  PRMT5

• UniProt:

  O14744

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human PRMT5 fragment

• Target:

  IBP; JBP; SKB; PRMT; HRMT1L; HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; PRMT5;72 kDa ICln binding protein;72 kDa ICln-binding protein; ANM5; Histone synthetic lethal 7; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase like 5; homolog of; Jak-binding protein 1; JBP 1; PRMT 5; Protein arginine methyltransferase 5; Protein arginine N methyltransferase 5; Protein arginine N methyltransferase 5 N terminally processed; Protein arginine N-methyltransferase 5; S. cerevisiae; S. POMBE; S. POMBE HOMOLOG OF; SHK1 KINASE BINDING PROTEIN 1; Shk1 kinase binding protein 1 homolog; Shk1 kinase/binding protein 1; Shk1 kinase-binding protein 1 homolog; SKB 1; SKB1 homolog; SKB1: SKB1 homolog (S. pombe)

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A338

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC; IP

• Field of Research:

  Epigenetics and Nuclear Signaling

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:2000-1:10000; IHC 1:50-1:100; IP 1:25-1:50

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  73 kDa

• Observed Molecular Weight:

  70 kDa

• Isotype:

  IgG, κ