Recombinant EGFR Monoclonal Antibody

• Background:

  EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.

• Abbreviation:

  EGFR

• UniProt:

  P00533

• Host:

  Rabbit

• Reactivity:

  Human; Mouse

• Immunogen:

  A synthetic peptide of human EGFR

• Target:

  Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) ; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; ERBB; ERBB1; Errp; HER1; mENA; NISBD2; SA7; wa2; Wa5

• Clonality:

  Monoclonal

• Clone:

  R03-6B1

• Conjugation:

  Unconjugated

• Applications:

  WB; IF

• Field of Research:

  Cancer; Signal Transduction

• Purification:

  Affinity Purified

• Concentration:

  300 μg/mL

• Dilution:

  WB 1:1000-1:2000; IF 1:50-1:200

• Buffer:

  50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  134kDa

• Observed Molecular Weight:

  175kDa

• Isotype:

  IgG