Recombinant PCAF Monoclonal Antibody

• Background:

  Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Also acetylates non-histone proteins, such as ACLY, PLK4, RRP9/U3-55K and TBX5. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5. Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4. Acetylates RRP9/U3-55K, a core subunit of the U3 snoRNP complex, impairing pre-rRNA processing. Also acetylates spermidine. (Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.

• Abbreviation:

  PCAF

• UniProt:

  Q92831

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human PCAF fragment

• Target:

  KAT2B; CAF; P/CAF; PCAF

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A271

• Conjugation:

  Unconjugated

• Applications:

  WB; IP

• Field of Research:

  Cell Biology; Epigenetics and Nuclear Signaling; Microbiology

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:1000; IP 1:25-1:50

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  93 kDa

• Observed Molecular Weight:

  93 kDa

• Isotype:

  IgG, κ