Recombinant HMGB1 Monoclonal Antibody

• Background:

  Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V (D) J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury.

• Abbreviation:

  HMGB1

• UniProt:

  P09429

• Host:

  Rabbit

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  Recombinant human HMGB1 fragment

• Target:

  SBP; HMGB; High Mobility Group Protein; High mobility group protein B; HMG; HMG-1; HMG1; HMG3; SBP-1; HMGB1; Amphoterin; High Mobility Group Protein 1; High mobility group protein B1; High mobility group protein 1 (HMG-1) ; High mobility group protein 1; high mobility group protein B1; high-mobility group box 1; HMG1; HMG-1; HMG1DKFZp686A04236; HMG3; HMGB1; SBP-1; Chromosomal protein; DKFZp686A04236; High mobility group 1; High mobility group box 1; high-mobility group (nonhistone chromosomal) protein 1; HMGB 1; nonhistone; NONHISTONE CHROMOSOMAL PROTEIN HMG1; SBP 1; Sulfoglucuronyl carbohydrate binding protein

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A253

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC; FCM; IP

• Field of Research:

  Epigenetics and Nuclear Signaling

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:2000; IHC 1:200-1:1000; FCM 1:50-1:100; IP 1:25

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  25 kDa

• Observed Molecular Weight:

  25 kDa

• Isotype:

  IgG, κ