Recombinant Cyclin D1 Monoclonal Antibody

• Background:

  The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

• Abbreviation:

  Cyclin D1

• UniProt:

  P24385

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  Recombinant Human Cyclin D1 protein

• Target:

  Cyl; PRAD; U21B; CCND; B cell leukemia; G1/S specific cyclin D; Cyclin D; BCL; BCL1; D11S287E; PRAD1; U21B31; CCND1; Cyclin D1; B cell leukemia 1; BCL 1; Cd1; Cyl 1; G1/S specific cyclin D1; BCL1; D11S287E; PRAD1; U21B31; CCND1; Cyclin D1; cyclin D1

• Clonality:

  Monoclonal, Recombinant

• Clone:

  5A3

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC

• Field of Research:

  Cell Biology; Epigenetics and Nuclear Signaling; Cancer

• Purification:

  Protein A

• Concentration:

  0.2 mg/mL

• Dilution:

  IHC 1:200-1:1000; WB 1:1000-1:5000

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  34 kDa

• Observed Molecular Weight:

  36 kDa

• Isotype:

  IgG, κ