LIGHT Antibody [7B9H9]

• Background:

  LIGHT Antibody: LIGHT, also known as Tumor Necrosis Factor Superfamily member 14 (TNFSF14), is a co-stimulatory molecule that can regulate T-cell activation (1) and has recently been identified as an immune checkpoint protein. LIGHT binds to two different receptors, Herpes Virus Entry Mediator (HVEM) and Lymphotoxin beta Receptor (LTβR) . While LIGHT binding to HVEM delivers a co-stimulatory signal to T cells (1), LIGHT binding to LTβR is critical for the formation of lymphoid structures which can stimulate T cell infiltration and activation of a tumor microenvironment, leading to rapid T cell-mediated tissue destruction (2) . It has been shown that targeted delivery of LIGHT to tumors, thereby causing the T cell infiltration of the tumor, can enhance the response of the PD-1/PD-L1 checkpoint blockade anti-cancer therapy (3), suggesting that LIGHT may become a potent tool in anti-cancer treatment.

• NCBI Gene ID:

  8740

• Swiss Prot:

  O43557

• Accession Number:

  NP_003798

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  LIGHT antibody was raised against the extracellular domain of human LIGHT.

• Clonality:

  Monoclonal

• Clone:

  7B9H9

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  LIGHT Antibody is supplied as protein A purified IgG2b.

• Positive Control:

  Cat. No. 1331 - Human Lymphoma Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  LIGHT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  LIGHT antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG2b

• Symbol:

  TNFSF14

• NCBI Official Name:

  TNF superfamily member 14

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wang Y, Zhu M, Miller M, et al. Immunoregulation by tumor necrosis factor superfamily member LIGHT. Immunological Reviews 2009; 229:232–43.

• Background Reference 02:

  Lee Y, Chin RK, Christiansen P, et al. Recruitment and activation of naive T cells in the islets by lymphotoxin beta receptor-dependent tertiary lymphoid structure. Immunity 2006; 25:499-509.

• Background Reference 03:

  Tang H, Wang Y, Chlewicki LK, et al. Facilitating T cell infiltration in tumor microenvironment overcomes resistance to PD-L1 blockade. Cancer Cell 2016; 29:285-296.

• Other Product Names:

  LIGHT Antibody: TNFSF14, LTg, CD258, HVEML, HVEM ligand

• Tested Applications:

  ELISA, WB, IHC-P, ICC, IF, Flow

• Protein ID:

  25952144

• Physical Properties:

  Liquid