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GAPDH Antibody [12D3H9]

CAT: 0223-PM-7571-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-PM-7571-01Size:0.02 mg
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Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1) . GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2) . GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
NCBI Gene ID
2597
Swiss Prot
P04406
Accession Number
NP_002037
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone
12D3H9
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Signal Transduction, Neuroscience, Homeostasis
Purification
GAPDH antibody is Protein A purified.
Positive Control
Cat. No. 1201 - HeLa Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (100%), Sheep: (93%), Bovine: (93%), Chicken: (93%), Rabbit: (93%)
Buffer
GAPDH antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
GAPDH antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Fragment
IgG
Specificity
GAPDH antibody is human, mouse, and rat reactive.
Symbol
GAPDH
NCBI Official Name
Glyceraldehyde-3-phosphate dehydrogenase
NCBI Organism
Homo sapiens
Background Reference 01
Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
Background Reference 02
Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.
Other Product Names
Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Tested Applications
ELISA, WB
Protein ID
7669492
Physical Properties
Liquid

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