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GAPDH Antibody [12D3D5]

CAT: 0223-PM-7569-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-PM-7569-01Size:0.02 mg
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Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1) . GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2) . GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
NCBI Gene ID
2597
Swiss Prot
P04406
Accession Number
NP_002037
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.
Clonality
Monoclonal
Clone
12D3D5
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Signal Transduction, Neuroscience, Homeostasis
Purification
GAPDH antibody is Protein A purified.
Positive Control
Cat. No. 1201 - HeLa Cell Lysate
Concentration
1 mg/mL
Buffer
GAPDH antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
GAPDH antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Fragment
IgG
Specificity
GAPDH antibody is human, mouse, and rat reactive.
Symbol
GAPDH
NCBI Official Name
Glyceraldehyde-3-phosphate dehydrogenase
NCBI Organism
Homo sapiens
Background Reference 01
Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
Background Reference 02
Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.
Other Product Names
Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Tested Applications
ELISA, WB
Protein ID
7669492
Physical Properties
Liquid

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