FADD (Phospho-Ser191) Antibody

• Background:

  The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

• Description:

  This is a FADD (Phospho-Ser191) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  MORT1 ; FAS-associating death domain-containing protein; Mediator of receptor induced toxicity

• Gene ID:

  14082

• Swiss Prot:

  Q61160

• Reactivity:

  Human, Mouse

• Immunogen:

  Peptide sequence around phosphorylation site of Serine191 (N-M-S (p) -P-V) derived from Mouse FADD.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Observed Molecular Weight:

  25kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG