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ENOS (phospho Thr494) Antibody

CAT: 0223-79-829Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0223-79-829Size:0.1 mg
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Background
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
NCBI Gene ID
4846
Swiss Prot
P29474
Accession Number
NP_000594.2
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
ENOS (phospho Thr494) antibody was raised against a peptide sequence around phosphorylation site of threonine 494 (R-K-K (p) -T-F derived from Human eNOS .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
140 kDa
Fragment
IgG
Specificity
The antibody detects endogenous levels of eNOS only when phosphorylated at threonine 494.
Applications Notes
Western Blot: 1:500~1:1000
Symbol
NOS3
NCBI Official Name
Nitric oxide synthase 3 (endothelial cell)
NCBI Organism
Homo sapiens
Background Reference 01
Janssens S.P., J. Biol. Chem. 267:14519-14522 (1992) .
Background Reference 02
Janssens S.P., J. Biol. Chem. 267:22694-22694 (1992) .
Background Reference 03
Marsden P.A., FEBS Lett. 307:287-293 (1992)
Other Product Names
CNOS, EC-NOS, ECNOS, NOS, NOS3
Tested Applications
WB
Protein ID
40254422
Physical Properties
Liquid

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