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ENOS (Ab 1177) Antibody

CAT: 0223-79-565Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0223-79-565Size:0.1 mg
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Background
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
NCBI Gene ID
4846
Swiss Prot
P29474
Accession Number
NP_000594.2
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
ENOS (Ab-1177) antibody was raised against a peptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
140 kDa
Specificity
This antibody detects endogenous level of total eNOS protein.
Applications Notes
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Symbol
NOS3
NCBI Official Name
Nitric oxide synthase 3 (endothelial cell)
NCBI Organism
Homo sapiens
Background Reference 01
Fulton, D. et al. (1999) Nature 399, 597-601. Harris, M. B. et al. (2001) J. Biol. Chem. 276, 16587-16591. Thomas, S. R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
Other Product Names
ENOS, ECNOS, Constitutive NOS, EC-NOS, NOS3, NOSIII
Tested Applications
WB, IF
Protein ID
40254422
Physical Properties
Liquid

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