Caspase 9 (phospho Thr125) Antibody

• Background:

  Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly (ADP-ribose) polymerase (PARP) . Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

• NCBI Gene ID:

  842

• Swiss Prot:

  P55211

• Accession Number:

  NP_001220.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Caspase 9 (phospho Thr125) antibody was raised against a peptide sequence around phosphorylation site of threonine 125 (P-E-T (p) -P-R) derived from Human Caspase 9.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  47 kDa

• Fragment:

  IgG

• Specificity:

  The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R) .

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  CASP9

• NCBI Official Name:

  Caspase 9, apoptosis-related cysteine peptidase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Duan H., J. Biol. Chem. 271:16720-16724 (1996) .

• Background Reference 02:

  Srinivasula S.M., J. Biol. Chem. 271:27099-27106 (1996) .

• Background Reference 03:

  Hadano S., Mamm. Genome 10:757-760 (1999)

• Other Product Names:

  APAF-3, MCH6, RNCASP9, CASP-9, ICE-LAP6

• Tested Applications:

  WB, IHC

• Protein ID:

  14790124

• Physical Properties:

  Liquid