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Caspase-9 Antibody

CAT: 0223-2073-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-2073-01Size:0.02 mg
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Background
Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα , TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
NCBI Gene ID
842
Swiss Prot
P55211
Accession Number
P55211
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
2073
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 1) shows similar CASPASE 9 expression profile in human cell lines detected by two independent anti-CASPASE 9 antibodies that recognize different epitopes, 2071 against N-terminus domain and 2073 against central domain. CASPASE 9 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2071 2073
Field of Research
Apoptosis, Cancer
Purification
Caspase-9 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1201 - HeLa Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Rat (50%) .
Buffer
Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Caspase-9 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Specificity
Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.
Isoform
Human CASPASE 9 has 4 isoforms, including isoform 1 (416aa, 46kD), isoform 2 (266aa, 30kD), isoform 3 (154aa, 17kD), and isoform 4 (333aa, 37kD) . Mouse CASPASE 9 has one isoform (454aa, 50kD) and Rat CASPASE 9 also has one isoform (454aa, 50kD) . 2073 can detect human and mouse isoform.
Symbol
CASP9
Positive Control 2
Cat. No. 17-004 - K-562 Cell Slide
NCBI Official Name
Caspase 9, apoptosis-related cysteine peptidase
NCBI Organism
Homo sapiens
Background Reference 01
Duan et al. J Biol Chem 1996;271:16720-4.
Background Reference 02
Srinivasula et al. J Biol Chem 1996;271:27099-106.
Other Product Names
Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9
Tested Applications
ELISA, WB, ICC, IF, IP
Protein ID
28558771
Physical Properties
Liquid

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