Products for Research Use Only

SPARC Antibody

CAT: 0223-79-734Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0223-79-734Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
NCBI Gene ID
6678
Swiss Prot
P09486
Accession Number
NP_003109.1
Host
Rabbit
Reactivity
Human
Immunogen
SPARC antibody was raised against a peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Stem Cell
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
42 kDa
Specificity
This antibody detects endogenous level of total SPARC protein.
Applications Notes
Western Blot: 1:500~1:1000
Symbol
SPARC
NCBI Official Name
Secreted protein, acidic, cysteine-rich (osteonectin)
NCBI Organism
Homo sapiens
Background Reference 01
Kelm R. J. Jr., Mann K. G. Blood 75:1105-1113 (1990) Wewer U. M., Albrechtsen R., Fisher L. W., Young M. F., Termine J. D. Am. J. Pathol. 132:345-355 (1988) Kuhn C., Mason R. J. Am. J. Pathol. 147:1759-1769 (1995)
Other Product Names
ON, ABL, JTK7, ON, BM-40
Tested Applications
WB
Protein ID
4507171
Physical Properties
Liquid