Doublecortin Antibody

• Background:

  Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.

• NCBI Gene ID:

  1641

• Swiss Prot:

  O43602

• Accession Number:

  NP_000546.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Doublecortin antibody was raised against a peptide sequence around aa.413~417 (P-T-S-P-G) derived from Human Doublecortin.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Stem Cell

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  40 - 45 kDa

• Specificity:

  This antibody detects endogenous level of total Doublecortin protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  DCX

• NCBI Official Name:

  Doublecortin

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Des Portes V., Pinard J. -M., Billuart P., Vinet M. -C., Koulakoff A., Carrie A., Gelot A., Dupuis E., Motte J., Berwald-Netter Y., Catala M., Kahn A., Beldjord C., Chelly J. Cell 92:51-61 (1998) Pilz D. T., Matsumoto N., Minnerath S. R., Mills P., Gleeson J. G., Allen K. M., Walsh C. A., Barkovich A. J., Dobyns W. B., Ledbetter D. H., Ross M. E. Hum. Mol. Genet. 7:2029-2037 (1998)

• Other Product Names:

  DC, DBCN, LISX, SCLH, XLIS, DCX, DC

• Tested Applications:

  WB

• Protein ID:

  30181246

• Physical Properties:

  Liquid