Ring1A Antibody

• Background:

  Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

• NCBI Gene ID:

  6015

• Swiss Prot:

  Q06587

• Accession Number:

  NP_002922.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Ring1A antibody was raised against a peptide sequence around aa.35~39 (I-A-V-S-P) derived from Human Ring1A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  58 kDa

• Specificity:

  This antibody detects endogenous level of total Ring1A protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  RING1

• NCBI Official Name:

  Ring finger protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Cao R., Tsukada Y., Zhang Y. Mol. Cell 20:845-854 (2005) Satijn D. P. E., Gunster M. J., van der Vlag J., Hamer K. M., Schul W., Alkema M. J., Saurin A. J., Freemont P. S., van Driel R., Otte A. P. Mol. Cell. Biol. 17:4105-4113 (1997)

• Other Product Names:

  RNF1, RING1A, RNF1, RING1

• Tested Applications:

  WB

• Protein ID:

  51479192

• Physical Properties:

  Liquid