PKM2 Antibody

• Background :

  Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

• NCBI Gene ID :

  5315

• Swiss Prot :

  P14618-1

• Accession Number :

  NP_872270.1

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  PKM2 antibody was raised against a peptide sequence around aa.405~409 (T-S-D-P-T) derived from Human PKM2.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Stem Cell

• Purification :

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration :

  1 mg/mL

• Buffer :

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight :

  60 kDa

• Specificity :

  This antibody detects endogenous levels of total PKM2 protein.

• Applications Notes :

  Western Blot: 1:500~1:1000

• Symbol :

  PKM

• NCBI Official Name :

  Pyruvate kinase, muscle

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Christofk, H. R. et al. (2008) Nature 452, 230-3. Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8. Dombrauckas, J. D. et al. (2005) Biochemistry 44, 9417-29. Hitosugi, T. et al. (2009) Sci Signal 2, ra73.

• Other Product Names :

  PK3, TCB, OIP3, PKM2, CTHBP, THBP1, HEL-S-30, CAGH44, TNRC10, PKM, PK3, PK2

• Tested Applications :

  WB

• Protein ID :

  33286420

• Physical Properties :

  Liquid