REST Antibody

• Background:

  Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

• NCBI Gene ID:

  5978

• Swiss Prot:

  Q13127

• Accession Number:

  NP_001180437.1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  REST antibody was raised against a peptide sequence around aa.873~877 (R-E-E-A-S) derived from Human REST.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  121 kDa

• Specificity:

  This antibody detects endogenous level of total REST protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  REST

• NCBI Official Name:

  RE1-silencing transcription factor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chong J. A. et. al. (1995) Cell 80:949-957Schoenherr C. J. et. al. (1995) Science 267:1360-1363Scholl T. et. al. (1996) J. Immunol. 156:1448-1457Lunyak V. V. et. al. (2002) Science 298:1747-1752

• Other Product Names:

  XBR, NRSF, RNF53, XBR, REST

• Tested Applications:

  WB

• Protein ID:

  301897977

• Physical Properties:

  Liquid