Synaptophysin 2 Antibody

• Background:

  Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1) . Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2) . Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3) . Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4) .

• NCBI Gene ID:

  66030

• Swiss Prot:

  P22831

• Accession Number:

  NP_076464.1

• Host:

  Rabbit

• Reactivity:

  Rat

• Immunogen:

  Synaptophysin 2 antibody was raised against a peptide sequence around aa.257~261 (G-P-T-S-F) derived from Rat synaptophysin 2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  37 kDa

• Specificity:

  This antibody detects endogenous levels of total synaptophysin 2 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  Synpr

• NCBI Official Name:

  Synaptoporin

• NCBI Organism:

  This antibody was raised against a peptide sequence around aa.257~261 (G-P-T-S-F) derived from Rat synaptophysin 2.

• Background Reference 01:

  Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4. Valtorta, F. et al. (2004) Bioessays 26, 445-53. Arthur, C. P. and Stowell, M. H. (2007) Structure 15, 707-14. Bonanomi, D. et al. (2007) Biochem J 404, 525-34.

• Other Product Names:

  Synpr

• Tested Applications:

  WB

• Protein ID:

  13027428

• Physical Properties:

  Liquid