GRASP Antibody

• Background:

  GRASP (GRP1-associated scaffold protein, tamalin) is a 395 amino acid proteinencoded by the human gene GRASP . GRASP is a scaffold protein that com-prises multiple protein-interacting domains, including a 95 kDa postsynapticdensity protein (PSD-95) /discs-large/ZO-1 (PDZ) domain, a leucine-zipperregion and a carboxyl-terminal PDZ-binding motif. GRASP is involved withintracellular trafficking and contributes to the macromolecular organizationof group 1 metabotropic glutamate receptors (mGluRs) at synapses. GRASPforms a heteromer composed of GRASP , PSCD2 and at least one mGluR-1. Italso interacts with PSCD3, mGluR-2, mGluR-3 and mGluR-5. GRASP is highlyexpressed in brain and has lower levels of expression in lung, heart, embryo, kidney and ovary.

• NCBI Gene ID:

  56850

• Swiss Prot:

  Q9JHZ4

• Accession Number:

  NP_064522.3

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  GRASP antibody was raised against a peptide sequence around aa. 814-818 (Q-E-I-V-R) derived from Rat GRASP.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  95 kDa

• Specificity:

  This antibody detects endogenous level of total GRASP protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  GRIPAP1

• NCBI Official Name:

  GRIP1 associated protein 1

• NCBI Organism:

  This antibody was raised against a peptide sequence around aa. 814-818 (Q-E-I-V-R) derived from Rat GRASP

• Background Reference 01:

  Kitano, J., Kimura, K., Yamazaki, Y., Soda, T., Shigemoto, R., Nakajima, Y. and Nakanishi, S. 2002. Tamalin, J. Neurosci. 22:1280-1289. Hall, B. S., Gabernet-Castello, C., Voak, A., Goulding, D., Natesan, S. K. and Field, M. C. 2006. J. Biol. Chem. 281: 27600-27612. Sugi, T., Oyama, T., Muto, T., Nakanishi, S., Morikawa, K. and Jingami, H. 2007. Crystal structures of autoinhibitory PDZ domain of Tamalin EMBO J. 26: 2192-2205.

• Other Product Names:

  GRASP-1, Gripap, Grasp, GRIP1-associated protein

• Tested Applications:

  WB

• Protein ID:

  46592991

• Physical Properties:

  Liquid