H2B Antibody

• Background:

  Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• NCBI Gene ID:

  8349

• Swiss Prot:

  P33778

• Accession Number:

  NP_003519.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  H2B antibody was raised against a peptide sequence around aa.41~45 (Y-V-Y-K-V) derived from Human H2B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  14 kDa

• Specificity:

  This antibody detects endogenous level of total H2B protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  HIST2H2BE

• NCBI Official Name:

  Histone cluster 2, H2be

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Borchert GM, et al. (2010) PLoS One. 5 (7) : e11641. Pennini ME, et al. (2010) PLoS Pathog. 6 (7) : e1000995. Lu C, et al. (2010) Sci China Life Sci. 53 (6) : 663-8.

• Other Product Names:

  H2B, H2BQ, GL105, H2B.1, H2BFQ, H2BGL105

• Tested Applications:

  WB

• Protein ID:

  4504277

• Physical Properties:

  Liquid