PLC gamma1 (phospho Tyr771) Antibody

• Background:

  PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor) -activated tyrosine kinase.

• NCBI Gene ID:

  5335

• Swiss Prot:

  P19174

• Accession Number:

  NP_002651.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  PLC-gamma1 (phospho-Tyr771) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 771 (P-D-Y (p) -G-A) derived from Human PLC-γ1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Neuroscience

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  155 kDa

• Specificity:

  This antibody detects endogenous level of PLC-gamma1 only when phosphorylated at tyrosine 771.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

• Symbol:

  PLCG1

• NCBI Official Name:

  Phospholipase C, gamma 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027. Margolis, B. et al. (1989) Cell 57, 1101-1107. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.

• Other Product Names:

  PLC1, NCKAP3, PLC-II, PLC148, PLCgamma1, CTNNB, OK/SW-cl.35, PRO2286, Phosphoinositide phospholipase C, Phospholipase C-gamma-1

• Tested Applications:

  WB, IF

• Protein ID:

  33598948

• Physical Properties:

  Liquid