Tau (phospho Ser214) Antibody

• Background:

  Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

• NCBI Gene ID:

  4137

• Swiss Prot:

  P10636

• Accession Number:

  NP_001116538.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Tau (Phospho-Ser214) antibody was raised against a peptide sequence around phosphorylation site of serine 214 (T-P-S (p) -L-P) derived from Human Tau.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Neuroscience

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  48, 62, 78 kDa

• Specificity:

  This antibody detects endogenous level of Tau only when phosphorylated at serine 214.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  MAPT

• NCBI Official Name:

  Microtubule-associated protein tau

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Smet C, et al. (2005) FEBS Lett; 579 (19) : 4159-64. Puig B, et al. (2005) Acta Neuropathol (Berl) . Gvtz J, et al. (2001) Science; 293 (5534) : 1491-5. Illenberger S, et al. (1998) Mol Biol Cell; 9 (6) : 1495-512.

• Other Product Names:

  TAU, MSTD, PPND, DDPAC, MAPTL, MTBT1, MTBT2, FTDP-17, BAF190A, BRG1, SNF2B, SNF2L4, MAPT, MTAPT, Neurofibrillary tangle protein, PHF-tau

• Tested Applications:

  WB

• Protein ID:

  178557734

• Physical Properties:

  Liquid